Puromycin Dihydrochloride 10mg/mL, 10 x 1 mL

Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces Alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.

Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. It is for use with eukaryotic selection or stable cell line generation application. The recommended working concentration ranges from 0.2-5.0 μg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/mL. Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.2-6.8) in 10 vials each containing 1 mL.