TBS Blocking Buffer, 1 L

TBS and PBS Blocking Buffer contain a single purified protein formulated in either Tris-buffer saline (TBS) or phosphate-buffered saline (PBS) for fast effective blocking of excess binding sites in western blots and ELISA assays. They are compatible with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. TBS/PBS blocking buffers do not contain albumin or endogenous biotin and therefore, are compatible with biotin/avidin systems. For ease of use, T20 (TBS or PBS) blocking buffers contain the detergent, Tween™ -20 at 0.05%, which can improve blocking performance in many western blot detection systems. TBS/PBS blocking buffer is a good choice to use when optimizing a new western blot system or as a replacement of milk-based blocking buffers exhibiting high background or masking antigen-antibody binding.