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EazeQuant Mouse IL-6 ELISA Kit, 96 Tests
EazeQuant Mouse IL-6 ELISA Kit is used to quantitively detect mouse Interleukin-6 (IL-6) in cell culture supernatant, serum and plasma.
Catalog number FB03233096
Price (CNY)
1,499.00
飞享价
2,258.82Save 759.82 (34%)
Each
Price (CNY)
1,499.00
飞享价
2,258.82Save 759.82 (34%)
Each
EazeQuant Mouse IL-6 ELISA Kit is used to quantitively detect mouse Interleukin-6 (IL-6) in cell culture supernatant, serum and plasma.
IL-6 is a cytokine encoded by the IL6 gene, plays a pivotal role in the maturation of B cells and inflammation, where the interleukin-6 is secreted into the serum, triggering a transcriptional inflammatory response through the interleukin 6 receptor alpha. Acting as an endogenous pyrogen, interleukin-6 can induce fever in individuals with autoimmune diseases or infections. Dysregulation of IL6 gene has been implicated in a wide array of inflammation-associated diseases, such as diabetes mellitus, systemic juvenile rheumatoid arthritis, and Kaposi sarcoma. Notably, elevated levels of the encoded protein have been observed in viral infections, including COVID-19 caused by SARS-CoV-2.
In the assay, samples, standards or controls are added to the microplate coated with the primary antibody, and then bind to the immobilized antibody in the microplate wells. Subsequently, the second antibody is introduced to the mixtures as the detector, followed by the addition of a substrate solution that generates signal to be measured. The intensity of this signal correlates to the concentration of mouse IL-6 in the original specimen.
IL-6 is a cytokine encoded by the IL6 gene, plays a pivotal role in the maturation of B cells and inflammation, where the interleukin-6 is secreted into the serum, triggering a transcriptional inflammatory response through the interleukin 6 receptor alpha. Acting as an endogenous pyrogen, interleukin-6 can induce fever in individuals with autoimmune diseases or infections. Dysregulation of IL6 gene has been implicated in a wide array of inflammation-associated diseases, such as diabetes mellitus, systemic juvenile rheumatoid arthritis, and Kaposi sarcoma. Notably, elevated levels of the encoded protein have been observed in viral infections, including COVID-19 caused by SARS-CoV-2.
In the assay, samples, standards or controls are added to the microplate coated with the primary antibody, and then bind to the immobilized antibody in the microplate wells. Subsequently, the second antibody is introduced to the mixtures as the detector, followed by the addition of a substrate solution that generates signal to be measured. The intensity of this signal correlates to the concentration of mouse IL-6 in the original specimen.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay Range31.3 - 2000 pg/mL
Assay Sensitivity6.5 pg/mL
Quantity96 Tests
Sample VolumeCell Culture Supernatant 50 µL, Serum 50 µL, Plasma 50 µL
Shipping Condition Wet Ice
Unit SizeEach
Contents & Storage
Contains:
• Mouse IL-6 Standard, Lyophilized (4 ng/mL upon reconstitution) 2 vials
• Coated Microwell Strips 1 pouch (12 strips with 8 wells each)
• Biotin — Conjugate (100X) 60 μL
• Streptavidin — HRP (100X) 150 μL
• Sample Diluent 12 mL
• Assay Buffer 18 mL
• Wash Buffer Concentrate (20X) 50 mL
• Substrate Solution (Tetramethyl — Benzidine) 15 mL
• Stop Solution (1M Phosphoric Acid) 15 mL
• Adhesive Film 4 pcs.
Store at:
2 — 8°C
• Mouse IL-6 Standard, Lyophilized (4 ng/mL upon reconstitution) 2 vials
• Coated Microwell Strips 1 pouch (12 strips with 8 wells each)
• Biotin — Conjugate (100X) 60 μL
• Streptavidin — HRP (100X) 150 μL
• Sample Diluent 12 mL
• Assay Buffer 18 mL
• Wash Buffer Concentrate (20X) 50 mL
• Substrate Solution (Tetramethyl — Benzidine) 15 mL
• Stop Solution (1M Phosphoric Acid) 15 mL
• Adhesive Film 4 pcs.
Store at:
2 — 8°C