DNase I, RNase-Free, 1 U/µL, 1000 U

DNase I is an endonuclease that digests both single- and double-stranded DNA by hydrolyzing phosphodiester bonds, resulting in mono- and oligodeoxyribonucleotides with 5’-phosphate and 3’-OH groups. The enzyme activity is strictly dependent on Ca²⁺ and is activated by Mg²⁺ ions. In the presence of Mg²⁺, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion.

Applications:

• Preparation of DNA-free RNA
• Removal of template DNA following in vitro transcription
• DNA labeling by nick-translation in conjunction with DNA Polymerase I
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting