T4 Polynucleotide Kinase, 10 U/µL, 2500 U

T4 Polynucleotide Kinase catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP, T4 Polynucleotide Kinase exhibits 5'-phosphatase activity and catalyzes the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction). The enzyme is also a 3'-phosphatase.

Applications:

• Labeling 5'-termini of nucleic acids

- Probes for hybridization
- Probes for transcript mapping
- Markers for gel electrophoresis
- Primers for DNA sequencing
- Primers for PCR

• 5'-phosphorylation of oligonucleotides, PCR products, other DNA or RNA prior to ligation
• Phosphorylation of PCR primers
• Detection of DNA modification by the [32P]-post labeling assay
• Removal of 3'-phosphate groups