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T4 DNA Ligase, 5 U/µL, 1,000 U
T4 DNA Ligase catalyzes phosphodiester bond formation between 5’-phosphate and 3’-hydroxyl termini in duplex DNA/RNA, repairing single-strand nicks and joining DNA fragments with either cohesive or blunt termini.
Catalog number FB02503100
Price (CNY)
152.00
飞享价
242.00Save 90.00 (37%)
Each
Price (CNY)
152.00
飞享价
242.00Save 90.00 (37%)
Each
T4 DNA Ligase catalyzes phosphodiester bond formation between 5’-phosphate and 3’-hydroxyl termini in duplex DNA/RNA, repairing single-strand nicks and joining DNA fragments with either cohesive or blunt termini. The reaction requires ATP as a cofactor.
Applications:
- Cloning of restriction enzyme generated DNA fragments.
- Cloning of PCR products.
- Joining double-stranded oligonucleotide linkers or adaptors to DNA.
- Site-directed mutagenesis.
- Amplified fragment length polymorphism (AFLP).
- Ligase-mediated RNA detection.
- Nick repair in duplex DNA, RNA or DNA/RNA hybrids.
- Self-circularization of linear DNA.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1,000 U
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Contains:
• T4 DNA Ligase, 5 U/µL 1000 U
• 10X T4 DNA Ligase Buffer 1.5 mL
• 50% PEG Solution 1.5 mL
Store at:
-25 — -15°C
• T4 DNA Ligase, 5 U/µL 1000 U
• 10X T4 DNA Ligase Buffer 1.5 mL
• 50% PEG Solution 1.5 mL
Store at:
-25 — -15°C