DNA Ligation Kit, 50 Rxn

DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature. The kit contains T4 DNA ligase, and a specially formulated 5X ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1-hour ligation. The ligation reaction mixture can be used directly for bacterial transformation.

Applications:

• Routine cloning experiments
• Blunt-end cloning
• Library construction
• TA cloning