E. Coli DNA Ligase, 400 U

E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalyzes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.
The E. coli DNA Ligase is active in wide temperature range (4°C to 37°C) and has high specificity to cohesive DNA ends.
Application includes nick ligation in double-stranded DNA, cDNA synthesis and cloning.