Thermosensitive Alkaline Phosphatase, 1,000 U

Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'- phosphate groups from DNA, RNA and nucleotides. This enzyme also removes phosphate groups from proteins. It's active in all Fisher Bioreagents SnapDigest restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends in 10 min at 37°C. The enzyme is inactivated in 5 min at 75°C. Therefore, removal of alkaline phosphatase is not required prior to ligation.

Applications:

• Dephosphorylation of cloning vector DNA to prevent recircularization during ligation.
• Simultaneous digestion and dephosphorylation of vector DNA.
• PCR product clean-up: nucleotide degradation prior to sequencing of PCR product.
• Dephosphorylation of nucleic acid 5'-termini prior to labeling with T4 Polynucleotide Kinase.
• Other applications where dephosphorylation of DNA and RNA substrates is necessary.
• Protein dephosphorylation.