SnapDigest NotI, 50 Rxn

5' G C ↓ G G C C G C 3'
3' C G C C G G ↑ C G 5'

SnapDigest NotI restriction enzyme recognizes GC^GGCCGC site and cuts best at 37°C in 5-15 minutes using universal SnapDigest Buffer.

SnapDigest enzymes are advanced restriction enzymes allowing rapid DNA digestion in just 5-15 minutes. They exhibit 100% activity in the universal SnapDigest and SnapDigest Green buffers, enabling simultaneous digestion with multiple enzymes in one tube without needing sequential steps. These enzymes work on plasmid, genomic, viral DNA, and PCR products without star activity, even during long incubations. Common downstream enzymes (for ligation, etc.) are also fully active in these buffers.

SnapDigest Green Buffer contains tracking dyes (blue ~3-5 kb, yellow <10 bp in 1% agarose) and a density reagent for direct gel loading. However, these dyes may interfere with fluorescence measurements, so the standard SnapDigest buffer is recommended for applications requiring fluorescence analysis.