TruScript Core H Minus RT Master Mix, 200 Rxn

TruScript Core H Minus RT Master Mix offers a convenient one-tube workflow for cDNA synthesis, optimized for two-step RT-qPCR applications. It includes TruScript Core H Minus Reverse Transcriptase (RT) and RNase Inhibitor. The RT enzyme, provides high thermostability, robustness, and increased cDNA synthesis rate. The RNase Inhibitor protects RNA from degradation at temperatures up to 55°C. The mix also contains reaction buffer, dNTPs, oligo(dT)18, and random hexamer primers. It supports reproducible cDNA synthesis from 1 pg to 1 µg of total RNA at 42-55°C, completing the reaction in 15-30 minutes.