TruScript Pro H Minus RT Master Mix with DNase, 50 Rxn

TruScript Pro H Minus RT Master Mix with DNase offers a convenient one-tube workflow for cDNA synthesis, optimized for two-step RT-qPCR applications. The kit includes a double-strand specific DNase (dsDNase) that removes contaminating genomic DNA from RNA samples in 2 minutes without affecting RNA quality or quantity. Its specific activity towards double-stranded DNA ensures RNA and single-stranded DNA (like cDNA and primers) remain intact, allowing dsDNase-treated RNA to be directly used in reverse transcription reaction. The master mix contains TruScript Pro H Minus Reverse Transcriptase (RT) and RNase Inhibitor. The RT enzyme, provides high thermostability, robustness, and increased cDNA synthesis rate. The RNase Inhibitor protects RNA from degradation at temperatures up to 55°C. The mix also contains reaction buffer, dNTPs, oligo(dT)18, and random hexamer primers. It supports reproducible cDNA synthesis from 1 pg to 2.5 µg of total RNA at 50-60°C, completing the reaction in 15-30 minutes.