TruScript Pro H Minus RT Master Mix, 50 Rxn

TruScript Pro H Minus RT Master Mix offers a convenient one-tube workflow for cDNA synthesis, optimized for two-step RT-qPCR applications. It includes TruScript Pro H Minus Reverse Transcriptase (RT) and RNase Inhibitor. The RT enzyme, provides high thermostability, robustness, and increased cDNA synthesis rate. The RNase Inhibitor protects RNA from degradation at temperatures up to 55°C. The mix also contains reaction buffer, dNTPs, oligo(dT)18, and random hexamer primers. It supports reproducible cDNA synthesis from 1 pg to 2.5 µg of total RNA at 50-60°C, completing the reaction in 15-30 minutes.