TruFidelis Plus Green PCR Master Mix, 500 Rxn

TruFidelis Plus Green PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. The master mix is ideal for applications where accuracy is important (cloning, sequencing, site directed mutagenesis). Two tracking dyes and a density reagent are pre-added to the master mix for direct loading of PCR products onto gels.
The master mix contains TruFidelis Plus DNA Polymerase, a high-fidelity, proofreading DNA polymerase combining a novel enzyme with a processivity-enhancing domain. It features a hot start mechanism, allowing room temperature reaction setup and storage of pre-assembled PCR reactions.

• 5´→3´ DNA polymerase activity
• 3´→5´ exonuclease activity
• Generates blunt-end amplification products
• Amplifies up to 10 kb from genomic DNA and up to 20 kb from low complexity DNA
• > 100X fidelity compared to Taq polymerase