2X TruTaq Green PCR Master Mix, 200 Rxn

TruTaq Green PCR Master Mix is a ready-to-use solution containing TruTaq DNA polymerase, optimized TruTaq Green buffer, MgCl2 , and dNTPs. It includes a density reagent and two tracking dyes for monitoring electrophoresis progress: The blue dye migrates with 3-5 kb DNA fragments in a 1% agarose gel and the yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have absorption peaks at 424 nm and 615 nm. TruTaq Green PCR Master Mix retains all the features of TruTaq DNA Polymerase. It is compatible with downstream workflows such as DNA sequencing, ligation and restriction digestion.

Applications:

• Routine PCR amplification of DNA fragments up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• RT-PCR
• Genotyping
• Generation of PCR products for TA cloning